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With an up gene amplitude threshold of 0.40 (equivalent to a 1.5-fold induction) and a down gene amplitude threshold of 0.40 (equivalent to a 1.5-fold repression), we discover that 76 genes lay above the up threshold and 6 lay below the down threshold

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As for the PV3 variants, S71pAcF and N77pAcF had Tm values of 58.7 C and 60.0 C, 6.7 C and 8 C higher than that of wild-type PV3