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% MPE (% maximal possible effect) = (control group flinch number of times - experimental group flinch number of times) 100 / control group flinch number of times Statistical analysis was carried out by one-way analysis of variance (ANOVA) with a post hoc test

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Higher levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) were found to be present, which is a marker for oxidative DNA damage, and when melatonin was introduced, there was an appreciable decrease in the levels of 8-OHdG, which shows that melatonin is a protective factor against ROS-induced DNA injuries (189)