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RNA isolation and quantitative RT-PCR Total RNA from cells was isolated with Trizol reagent (Qiagen GmbH, Hilden, Germany) and RT-PCR was conducted using a quantitative SYBR Green RT-PCR kit (Applied Biosystems, Warrington, UK) was used with a Step One Plus RT-PCR System (Applied Biosystems)

Can cause scalp irritation, unwanted facial hair, and rare cardiovascular effects

Esto se debe a que el ADN del espermatozoide aporta la mitad de la informacin gentica del embrin

Shaikh NA, Zhang XB, Abdalla MI, Baylink DJ, Tang X

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