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It echoed the concerns that led to sibutramine's withdrawal from the market in 2010, making regulators understandably cautious

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466 These findings suggest that modulation of microglial bioenergetic pathways and metabolic state might be promising strategies to treat AD

Sections were deparaffinized, rehydrated, stained with hematoxylin for 8 min, rinsed, differentiated in acid alcohol for 30 s, blued for 2 min, counterstained with eosin for 2 min, dehydrated, cleared, and mounted with neutral resin (Solarbio, G1120)

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Tail vein samples were obtained for immediate glucose measurement as above, or into lithium heparin-coated microvette tubes for plasma insulin measurement using a mouse insulin-specific HTRF (Cisbio)