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cAMP accumulation assay Wild-type GLP-1R and GLP-1R mutants were cloned into the expression receptor pcDNA3.1/V5-His-TOPO vector (Invitrogen) at the HindIII and EcoRI sites by using the QuickChange site-directed mutagenesis and a flag-tag was inserted after native signal sequence (see primers in Supplementary Table 2)

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