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Phosphorylation assay was performed in the kinase buffer (0.6% (w/vol) CHAPS, 150 mM NaCl, 25 mM Hepes pH 7.5, 10 mM MgCl 2 and 1 mM MnCl 2 supplemented with 0.25 mM ATP) and incubated at 25 C for 5 min

10.1016/j.molcel.2012.10.007 74 WongC

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You know, again, that's, that's why this is in the gray area, but I will, there's, like I said, there's part of me that wants to then after six weeks, go back and, and train like more than I had been and see if it makes a difference

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