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For surface downregulation, labeling was performed with SNAP-Surface-488 in MIN6B1-SNAP-GLP-1R cells before fixation, or with anti-rodent GLP-1R (2 g l 1 ) in fixed MIN6B1 and INS-1 832/3 cells plus FITC-conjugated anti-mouse secondary (F0257, Sigma, 1/200)

Interestingly, DSIP doesn't bind directly to opioid receptors like morphine or endorphins

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References: Intermittent pneumatic compression therapy: a systematic review Effects of Intermittent Pneumatic Compression on Delayed Onset Muscle Soreness (DOMS) in Long Distance Runners A systematic review of pneumatic compression for treatment of chronic venous insufficiency and venous ulcers Effects of lower-limb intermittent pneumatic compression on sports recovery: A systematic review and meta-analysis Effects of daily use of intermittent pneumatic compression in competitive handball players: A randomized controlled trial Infrared therapy for chronic low back pain: a randomized, controlled trial Effects of local vibration therapy on various performance parameters: a narrative literature review Long-term results of vibratory stimulation as a pain relieving measure for chronic pain Assessment of Countermovement Jump: What Should We Report

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