4050 g of plasmid DNA was mixed with 200 l of 2.5 M CaCl 2 and 1.7 ml H 2 O, after which 2 ml of 2xHBS buffer (50 mM HEPES pH 7.05, 140 mM NaCl, 1.5 mM Na 2 HPO 4 2H 2 O) was added dropwise while vortexing or bubbling the solution to enable formation of precipitates
About Reta in Research
Usage: 2 drops twice a day Specification: Material:Water, coffee extract, green tea extract, etc
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Gastroenterology 132:21312157 Todd JF, Wilding JP, Edwards CM, Khan FA, Ghatei MA, Bloom SR (1997) Glucagon-like peptide-1 (GLP-1): a trial of treatment in non-insulin-dependent diabetes mellitus