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Detection of primary antibodies was performed with Alexa Flour 488 anti-rabbit (A11034) and Alexa Fluor 633 anti-mouse (A21052) secondary antibodies (1:200) (Invitrogen)

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Key Findings: GLP-1 Release: Oral administration of D-allulose stimulates the release of GLP-1, a hormone known for its appetite-suppressing and glucose-regulating properties