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After that, all the culture media was replenished by 100 L DMEM medium and 20 L MTS reagent (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt)

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Iodoacetamide was added to 10 mM and incubated in the dark at room temperature for 30 min to alkylate the proteins

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