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The beads were then washed three times with 1 ml of RIPA buffer, followed by washes with 1 M KCl, 0.1 M Na 2 CO 2 and 2 M urea in 10 mM Tris-HCl (pH 8.0)

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Rooibos utilises transpirational leaf cooling during summer and has the ability to alter chlorophyll concentrations and to accumulate phenolic compounds in the leaves, all to decrease the effects of heat stress (MacAlister et al

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It can show if there are problems with the rotator cuff, the labrum, or the portion of the biceps tendon that is inside the shoulder joint