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Vero cells were infected with PEDV and the maintenance medium was replaced for 5 hours followed by erastin-supplemented medium, and the supernatant and pellet were collected at 37C for 2 hours to extract viral supernatant nucleic acid to assess the release of PEDV and extract intracellular RNA to assess the content in viral cells

It is a form of sorbic acid, a naturally occurring fatty acid which is easily metabolized in the body

For the next perhaps 12 to 24 hours, which is the second phase of the so-called inactive (latent) phase, the patient feels well

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