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The primary antibodies that were used were as follows: antibodies against cardiac troponin T (#ab209813, 1: 3000 for cardiomyocytes), antibodies against Col11 (#ab270993, 1:2000), antibodies against Vimentin (#ab24525, 1:300) and cardiac troponin T (#ab8295, 1: 2000 for myocardial tissue) were obtained from Abcam (Cambridge, UK)

The same team has previously reported that doxycycline-induced downregulation of IGF-1R in mammary epithelium using the same animal model induced tumor regression [92]

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Instead, it influences melanin production and can lead to a more even skin tone over time