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Several RS animal model studies also indicated functional, morphological, and molecular changes in synapses in the brain

Freezing causes ice crystal formation, which physically disrupts amino acid structure and denatures dissolved compounds

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A complementary axis involves transcriptional reinforcement of antioxidant programs, including NFE2 like BZIP transcription factor 2 (NFE2L2, also known as NRF2) signaling, which increases GPXs and further suppresses ROS-driven inflammasome activation 58

The VPA-exposed group exhibited intense Iba1 immunostaining, indicating pronounced proinflammatory microglial activation compared to controls (P 0.05)

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