Preclinical in vivo models have examined its effects on nitric oxide synthase activity, extracellular matrix protein expression, and actin cytoskeletal dynamics in connective tissue contexts
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The samples were fixed with 4% PFA at room temperature for 30 min, permeabilized with 0.1% Triton-100 at room temperature for 15 min, and blocked with 5% BSA solution at 37 C for 1 h
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Dinner: Lentil soup with leafy greens and whole-grain bread
In addition, transfection of GST into recipient cells increased their sensitivity to the drug (Rosario et al ., 2000)