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Production of peroxynitrite after MDMA exposure likely leads to the oxidative modifications of some mitochondrial complex proteins, resulting in their inhibition, as described [48, 49]

After 24 hours of incubation at 37C, colony counts were performed to quantify surviving bacteria

The injection devices are also not identical

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Cells were subsequently mounted with PermaFluor and visualized using an inverted fluorescence microscope (Nikon Eclipse Ti) at 20 or 40 magnification