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However, the RNA-seq approach employed cannot assess the level of small RNAs (less than ~100nt), and a number of small RNA genes were identified as containing germline LAG-1 ChIP-seq peaks (e.g, mir-61/250 , five 21 U RNA genes)

Measurement of early and late apoptosis using annexin V and PI flow cytometry We used FITC Annexin V Apoptosis Detection Kit with PI (BioLegend, San Diego, CA) to quantitate the early and late apoptosis of RINm5F cells

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healthcare organizations, covering the period from April 2005 to April 2025

A key challenge in studying native GLP-1 is its extremely short in vivo half-life of approximately 12 minutes, owing to rapid inactivation by dipeptidyl peptidase-4 (DPP-4), which cleaves the N-terminal His-Ala dipeptide from GLP-1 to produce the inactive metabolite GLP-1(936)amide