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The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 g/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 g/mL) (Bio-Sciences) for 30 min protected from light, at room temperature

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Storage and Shelf Life Unreconstituted (lyophilized) peptides are stable at room temperature for shipping but should be refrigerated (2-8 C / 36-46 F) upon arrival

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Phase 2b clinical trials were completed in Australia testing the drug in 300 obese individuals

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