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These findings were supported by the relaxation of large vessels (ex vivo), mainly by acting on vascular endothelial cells but also on vascular smooth muscle cells, increasing NO production and migration of endothelial cells, activating the signal pathways of Src, Cav-1, and eNOS, and reducing proteinprotein interaction between eNOS and Cav-1 [121,122]

This high flexibility is also notable in the two-dimensional classes of the prefusion conformation side views that show no clearly defined density for the membrane disc (Extended Data Fig

Taken together, these heterogeneous findings highlight both biological plausibility and the methodological constraints that shape the current evidence base, motivating the integrative Discussion below