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Then, the inserts containing cells were placed into 24-well plates containing endothelial culture medium

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2.8 Mass spectrometry analysis Peptides were injected into a Nano UHPLC Ultimate 3,000 system (Thermo Fisher Scientific) connected to Exploris 480 Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific)

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The green curve shows the NAC rescue of the male by the protocol described in the text starting The black curve in Figure 5 shows the response of the male to an 18-gm dose of APAP if not treated