For serum samples, 75 L of chloroform:methanol extraction solvent (v:v = 1:2) was added to 20 L of mouse serum, with the exception of the serum from WT, Gclc L-KO, Nrf2 L-KO, and L-DKO mouse serum, for which 20 L of mouse serum was combined with 180 L of chloroform:methanol extraction solvent (v:v = 1:2) containing internal standards at the final concentrations: 5 nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5 nM D3-Deoxysphinganine (Avanti Polar Lipids Inc., Cat# 860474), and SPLASH LIPIDOMIX (1:1000, Avanti Polar Lipids Inc., Cat# 330707)
Uremic toxicity-induced eryptosis and monocyte modulation: the erythrophagocytosis as a novel pathway to renal anemia
Free GSH concentration in the sample is calculated from the difference between two separate measurements the total GSH measurement and a measurement of the GSH generated from GSSG
A clinical peptide serum designed to reduce the appearance of fine lines and wrinkles, improve firmness, and support long-term skin repair using copper peptides backed by research
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Glutathione in Protein Redox Modulation through S-Glutathionylation and S-Nitrosylation