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Ubiquitination assays comparing *NSD2 PWWP1 mutants were performed similarly, except that SKP1-FBXO22, *NSD2 PWWP1 mutants, and compounds were not incubated separately prior to the start of the reaction
Nat Rev Mol Cell Biol 13(10):607625 Fernandes MA, Marques RJ, Vicente JA, Santos MS, Monteiro P, Moreno AJ et al (2008) Sildenafil citrate concentrations not affecting oxidative phosphorylation depress H 2 O 2 generation by rat heart mitochondria