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Anabolic signaling was determined as the quantity of pAKT and pRPS6 normalized to Glycerinaldehyde-3-phosphate-Dehydrogenase (GAPDH)

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(PubMed) Hurwitz BE, Klaus JR, Llabre MM, et al

PCR was performed using PCR Master Mix kit (Fermentas, USA) in a final volume of 50 l

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