Storage shortcuts room temperature storage, repeated freeze-thaw cycles of reconstituted solution, extended use past 28-day reconstituted shelf life introduce variables that compromise research integrity
If a reducing-agent-containing assay buffer is required, add GHK-Cu in the final step at the working dilution
doi: 10.1007/s00439-005-1255-6
The Apollo-NADP + sensors are fluorescently tagged enzymatically inactive G6PD constructs that enable NADPH/NADP + redox states measurement through fluorescence anisotropy (Fig 1B) [23]
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94 SaitoT.MatsubaY.MihiraN.TakanoJ.NilssonP.ItoharaS.et al (2014)