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It is also noteworthy that Day 21, or around this timepoint, has been identified as an important stage for liver organoid functional development in several previous studies 13,15,28,29

In certain bacteria, such as Eubacterium hallii and Anaerostipes caccae , acetate and lactate can be converted into butyrate [15, 16]

PubChem CID: 16132341

In contrast, we found that these metabolomic alterations led to a reduction in the plasma level of 66 different metabolites in acute patients versus the R group ( Supplementary Figures 9A-C )

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