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Pull-down assay and immunoblotting Interactions between exendin 939 and TRPV1 were examined via His-mediated pull-down assays using a modified protocol from the Pierce Pull-down PolyHis Protein:Protein Interaction Kit (Thermo Fisher Scientific)

Three rounds of review were required to establish consensus

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In a further set of experiments, IBMX (at a higher dose) was added only for the final 10 min out of the 16 h incubation for a point estimate of cAMP synthesis rate