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This includes those who have giant congenital pigmented naevi (benign melanocytic naevi originating in utero measuring greater than 20 cm in diameter [47]), a family history of three or more melanoma cases, more than 100 normal moles, or any atypical moles (particularly if multiple) [24]

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Flow cytometry data were pre-processed as follows: fluorescence data were scaled and checked for uniform positive and negative events expression