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The initial PCR reaction was carried out at 94 C for 5 min, 15 cycles of 94 C for 30 s, 56 C for 30 s and 72 C for 90 s, with a final extension of 72 C for 10 min

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Dysregulation of BAs has been shown in individuals with major depressive disorder (MDD), where increased levels of 23-nordeoxycholic acid, a DCA derivative, were positively correlated with depression scores 47