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Glutathione also promotes the optimal function of detoxification pathways

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Western blotting Sample preparation Liver tissues were pulverized in liquid nitrogen and 20 mg were weighed and homogenized in ice cold 1X RIPA buffer (50 mM Tris, 150 mM sodium chloride (NaCl), 1% NP40, 0.5% sodium deoxycholate and 0.1% SD, pH 8) using a glass Dounce homogenizer

Key finding: NNMT positioned as a master metabolic regulator at the intersection of NAD+ salvage, one-carbon metabolism, and epigenetic control