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Am J Pathol (2005) 166(2):43342

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41 : 461499 Debatin KM (1996) Disturbances of the CD95 (APO-1/Fas) system in disorders of lymphohaematopoietic cells

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FITC-agonist was added for 30 min at 37 C to induce internalization, then removed and washed with cold HBSS to arrest further endocytosis, and cells treated with 500 mM ice-cold sodium 2-sulfanylethanesulfonate (MesNa), a membrane-impermeable reducing agent, in alkaline TNE buffer (100 mM NaCl, 50 mM Tris-HCl, pH 8.6) to cleave BG-SS-biotin specifically from cell surface receptors