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GSSG is reduced back to GSH by the enzyme glutathione reductase, which also requires NADPH as a cofactor
220 (1), 57.e1e13
The reduction in hepatic GSH after a therapeutic dose is minor, but a 15 g dose almost completely depletes hepatic GSH between 2 and 10 hours
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147,148 In one placebo-controlled trial in 46 men with low sperm numbers, those who received ashwagandha root extract (standardized to contain at least 5% withanolides), at 225 mg three times daily for 90 days, had a 167% increase in sperm number, 57% increase in sperm motility, and 53% increase in semen volume, while those who received placebo had little change in these parameters
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