In brief, serial dilutions of G49 and Fc-GLP1 in assay buffer (as above) were added in duplicate to CHO cells recombinantly expressing human GLP-1R and incubated for 30 min at RT before detection with the cAMP dynamic 2 HTRF assay
PPAR- increases lipoprotein lipase gene expression, and down-regulates hepatic apolipoprotein C-III in humans (Gervois et al., 2000)
(ii) E2 group: the cells were treated with 10 8 mol/L E2
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The predicted translated DNA sequence revealed a 205 amino acids protein with a predicted molecular weight of 24.04 kDa