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Briefly, proteins were subjected to sodium dodecyl sulfate-10% polyacrylamide gel electrophoresis (SDS-10% PAGE) under reducing conditions in Laemmlis buffer system followed by transfer to nitrocellulose membranes 19

Rodrigues C, Milkovic L, Bujak IT, Tomljanovic M, Soveral G, Cipak Gasparovic A

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However, results depend on consistency, bioavailability, and supportive nutrients, not just milligrams alone

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