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Khotskaya YB, Holla VR, Farago AF, Mills Shaw KR, Meric-Bernstam F, Hong DS (2017) Targeting TRK family proteins in cancer

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Full benefits may take several months, especially for protocols targeting muscle gain, weight loss, or hormone regulation

After pharmacological intervention in 96-well cell culture plates for 24, 48 or 72 h, the culture medium was removed and replaced with 0.1 mg MTT reagent (Beyotime, ST1537) per well dissociated in 100 L basal DMEM (Procell, PM150210)

Interestingly, this tamoxifen-induced differentiation occurs in a PKC signaling independent-manner, and not only by activation of classical estrogen receptors but also by activation of GPER-1 (Gonzalez et al., 2016)

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