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Then, 100 l of substrate (tetramethylbenzidine) was added to each well after washing and incubated at room temperature for 30 min

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For immunofluorescence staining, tissues or cells were first fixed with 4% paraformaldehyde and then permeabilized using 0.1% Triton X-100 in PBS to facilitate antibody penetration into cells

Effect of active modified atmosphere packaging with different initial gas compositions on nutritional compounds of shiitake mushrooms ( Lentinus edodes )

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DHA can undergo irreversible hydrolysis to 2, 3-diketogulonic acid, if not reduced by DHAR