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Protein bands were visualized using WesternBright Quantum (Advansta, Menlo Park, California, USA) and quantified by a ChemiDoc XRS + system with Image Lab software (Bio-Rad, Hercules, CA, USA)

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doi: 10.1016/j.xcrm.2021.100387 104 ArodaVRAberleJBardtrumLChristiansenEKnopFKGaberySet al

Prepare in a sterile environment: The individual administering the injection should use an alcohol swab to clean the injection site and wash their hands thoroughly

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Teams scoping a comparative protocol may find our retatrutide vs semaglutide vs tirzepatide breakdown useful for study-design context