"Glutathione-Sensitive Nanogels for Drug Release"
All cells were cultured in an endothelial cell-specific medium for human umbilical vein endothelial cells, which was supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (PCM-H-040, ZQXZbio, China)
Brix, M
Some may notice changes in skin texture and hydration within 12 weeks, but collagen remodeling and deeper changes may take 412 weeks with consistent use
Cells were lysed for 15 minutes in ice-cold RIPA buffer consisting of 1% NP40, 0.1% SDS, 0.5% deoxycholate in PBS, supplemented with 1 mM PMSF, 1X cOmplete, EDTA-free Mini Protease Inhibitor Cocktail (Sigma Cat#11873580001) and 1X PhosSTOP (Sigma Cat#4906845001)
Mao R, Zong N, Hu Y, Chen Y, Xu Y