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Boxplots showing the concentrations (y-axis) of the significantly altered metabolites in control (n = 9), compared to 50 (n = 9), 35x6 (n = 9), and 4+1 (n = 9) treatment groups analyzed by one-way ANOVA, p-value 0.1)

Glutathione only unfolds its full potential in conjunction with its co-factors

HPLC methods with either UV or fluorescence detection require derivatisation of GSH prior to detection which causes further limitations driven principally as a result of enhanced susceptibility to GSH degradation and auto oxidation, instability of derivatised GSH adduct and suboptimal derivatisation conditions

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