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Nutritional and Lifestyle Support Restoring copper-zinc balance is about improving the bodys natural regulation rather than forcing levels down abruptly

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Cells were centrifuged and resuspended in 0.1 M sodium cacodylate buffer and post-fixed for 1 h with 1% osmium + 0.1 M sodium cacodylate buffer, then dehydrated and embedded in Agar 100 resin

A two-gradient mobile phase (A: water + 0.1 % formic acid and B: acetonitrile + 0.1 % formic acid) and a flow rate of 0.3 mL min-1 was used