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To determine occurring in-source fragments, which increase the sample complexity without yielding significant additional information, MS/MS spectra were acquired using the same conditions with a collision energy ramp between 2.00 and 4.00 eV

The accumulation of extracellular lactate and its metabolites can inhibit T-cell motility, potentially limiting their infiltration into sites of inflammation and modulating immune responses

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Intracellular calcium Fluo4/AM or X-Rhod-1, AM [398]

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Which one applies depends on the protocol page being followed always match the dose to the vial