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The HeLa cells were grown overnight on a plasma-treated 25 mm round cover slip mounted onto a 35 mm Petri dish with an observation window

Centrifugations were repeated for a total of three spins to clear sufficient fat from the samples

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However, ghrelin did not alter dopamine binding potential (reflecting changes in endogenous tone), but boosted task-free phasic pulses of the NAcc BOLD signal ( p = .020), which were negatively associated with binding potential ( p = .034)