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Next, the protein was eluted using LDOX elution buffer (20 mM potassium phosphate, 200 mM NaCl, 2.5 mM d -desthiobiotin, pH 7.4) and the fractions were combined according to the recorded absorption 280 nm ( A 280nm )

Through detailed molecular analysis, LC-MS enables scientists to detect sequence variants, degradation products, and process-related impurities that may arise during peptide synthesis or formulation

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Initially, orally administered vitamin B12 is tightly bound to the protein Haptocorrin (HC), encoded by the TCN1 gene, which is released into saliva to protect it from active, harmful factors, such as HCl, in the stomach and intestines [98, 99]