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Cells were seeded in 96-well plates at a density of 35,000 cells per well and transiently transfected using Lipofectamine 2000 according to the manufacturers protocol
Dokken BB, Henriksen EJ
Res Rev Biomed Biotech 1(2):8389 Kiani AK, Pheby D, Henehan G, Brown R, Sieving P, Sykora P, Marks R, Falsini B, Capodicasa N, Miertus S (2022) Ethical considerations regarding animal experimentation
Poczenie obu ramion daje w modelach silniejsze, zachowujce fizjologiczn pulsacyjno uwalnianie GH ni pobudzenie pojedynczego szlaku
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0.9% in controls) ( Table 1 )
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