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To each tissue sample collected from the limbs, weighing between 40 and 60 mg, 1 mL of H2O and 2 mL of cold methanol were added with the aim of halting enzymatic metabolism (quenching)

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Inhibiting system Xc reduces GSH production, thereby diminishing the activity of GPX4 and leading to ROS accumulation, ultimately driving ferroptosis in CRC cells [61, 69-72]

Response mechanism of probe TPE-4NE-O and FA-DSPE/TPE-4NE-O response to the hypoxic tumor