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discovered that MnCl 2 treatment to SH-SY5Y, a common cell model for dopaminergic neurons, resulted in mitochondrial membrane potential loss, ROS production, and apoptosis (Lee et al., 2009)

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Obviously, the rate of extraction medium replenishment of a given process is a variable which is easily determined at the time the process is performed and, therefore, no precise limits for the rate must be predetermined

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