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Mammalian frataxin directly enhances sulfur transfer of NFS1 persulfide to both ISCU and free thiols

Each freeze-thaw cycle creates ice crystals that can physically disrupt peptide structure, and the process concentrates the peptide at the boundary between frozen and unfrozen regions, potentially causing aggregation

Hydrogen water may help compensate for this decline by providing alternative antioxidant protection

Sterile water lacks a bacteriostatic agent

Some individuals find daily administration easier to remember and integrate into their routine than weekly injections that require planning