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To assemble the GIPRG s complex, 15 M tirzepatide (GL Biochem) was added to the preparation accompanied by 100 M TCEP, 25 mU/mL apyrase (Sigma-Aldrich), 20 g/mL Nb35 and 100 U salt active nuclease (Sigma-Aldrich) supplemented with protease inhibitor cocktail for 1.5 h incubation at room temperature (RT)